Validating microarray data interracial dating hispanic and white
Validating Microarray Data Using RT 2 Real-Time PCR Products Introduction: Real-time PCR monitors the amount of amplicon as the reaction occurs.
Usually, the amount of product is directly related to the fluorescence of a reporter dye.
Most real-time systems detect and accommodate SYBR Green making the method very flexible; however, some instrumentation may also require the simple addition of a reference dye to normalize the system s optics.
SYBR Green specifically binds double-stranded DNA by intercalating between base pairs, and fluoresces only when bound to DNA.
Figure 1: Induction of TNFAIP3 gene expression in He La cells upon TNF-alpha treatment.
Total RNA was isolated from He La cells that were either treated with TNF-alpha (20 ng/ml, 30 min) or left untreated.
Each PCR master mix features ready-to-use convenience.